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Post by samuel on Jan 3, 2021 17:18:47 GMT -5
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Post by samuel on Jan 3, 2021 17:24:29 GMT -5
. Results
For Nakht-Ankh, mtDNA enrichment yielded 5,707,546 sequence
reads, of which 6138 mapped to the rCRS (Andrews et al., 1999). Forty
SNPs were observed with a mean coverage of 18.3×, spanning the
entire mtDNA (Table 1). Shotgun sequencing gave 1488 reads mapping
to the rCRS covering 30 polymorphic sites with 2–14× coverage, in
each case agreeing with the SNP identified from the enriched sample.
For Khnum-Nakht, 76,253,577 reads were obtained from the enriched
library, 5422 mapping to the rCRS, revealing the same forty SNPs as
typed for Nakht-Ankh, with 15.5× mean coverage (Table 1). No high
quality reads were obtained from the shotgun data for Khnum-Nakht.
The SNP identities were consistent with mtDNA haplogroup M1a1 with
88.05–91.27% degree of confidence, thus confirming the African ori-
gins of the two individuals. The haplotypes included ancestral muta-
tions at positions 489 T → C, 10,398A → G, 10,400C → T an
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