Post by djoser-xyyman on Aug 20, 2011 4:27:43 GMT -5
This will answer a lot of questions. This is from Australia. . .
Quote:
"Ancient mitogenomics
Purchase
References and further reading may be available for this article. To view references and further reading you must purchase this article.
Simon Y.W. Hoa, 1, , and M. Thomas P. Gilbertb, 1,
aCentre for Macroevolution and Macroecology, Research School of Biology, Australian National University, Canberra ACT 0200, Australia
bCentre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, DK-2100 Copenhagen, Denmark
Received 8 July 2009; revised 21 September 2009; accepted 23 September 2009. Available online 27 September 2009.
Abstract
The mitochondrial genome has been the traditional focus of most research into ancient DNA, owing to its high copy number and population-level variability. Despite this long-standing interest in mitochondrial DNA, it was only in 2001 that the first complete ancient mitogenomic sequences were obtained. As a result of various methodological developments, including the introduction of high-throughput sequencing techniques, the total number of ancient mitogenome sequences has increased rapidly over the past few years. In this review, we present a brief history of ancient mitogenomics and describe the technical challenges that face researchers in the field. We catalogue the diverse sequencing methods and source materials used to obtain ancient mitogenomic sequences, summarise the associated genetic and phylogenetic studies that have been conducted, and evaluate the future prospects of the field.
Keywords: Ancient DNA; Post-mortem damage; Contamination; Polymerase chain reaction; High-throughput sequencing; Phylogenetics
Article Outline"
Quote:
"Ancient mitogenomics
Purchase
References and further reading may be available for this article. To view references and further reading you must purchase this article.
Simon Y.W. Hoa, 1, , and M. Thomas P. Gilbertb, 1,
aCentre for Macroevolution and Macroecology, Research School of Biology, Australian National University, Canberra ACT 0200, Australia
bCentre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, DK-2100 Copenhagen, Denmark
Received 8 July 2009; revised 21 September 2009; accepted 23 September 2009. Available online 27 September 2009.
Abstract
The mitochondrial genome has been the traditional focus of most research into ancient DNA, owing to its high copy number and population-level variability. Despite this long-standing interest in mitochondrial DNA, it was only in 2001 that the first complete ancient mitogenomic sequences were obtained. As a result of various methodological developments, including the introduction of high-throughput sequencing techniques, the total number of ancient mitogenome sequences has increased rapidly over the past few years. In this review, we present a brief history of ancient mitogenomics and describe the technical challenges that face researchers in the field. We catalogue the diverse sequencing methods and source materials used to obtain ancient mitogenomic sequences, summarise the associated genetic and phylogenetic studies that have been conducted, and evaluate the future prospects of the field.
Keywords: Ancient DNA; Post-mortem damage; Contamination; Polymerase chain reaction; High-throughput sequencing; Phylogenetics
Article Outline"